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KMID : 0923620140140040219
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Immune Network
2014 Volume.14 No. 4 p.219 ~ p.225
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Relationship between Poor Immunogenicity of HLA-A2-Restricted Peptide Epitopes and Paucity of Naive CD8＋ T-Cell Precursors in HLA-A2-Transgenic Mice
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Choi Yoon-Seok
Lee Dong-Ho
Shin Eui-Cheol
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Abstract
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We examined the immunogenicity of H-2 class I-restricted and HLA-A2-restricted epitopes through peptide immuniza-tion of HLA-A2-transgenic mice that also express mouse H-2 class I molecules. All four of the tested epitopes restricted by H-2 class I robustly elicited T-cell responses, but four of sev-en epitopes restricted by HLA-A2 did not induce T-cell re-sponses, showing that HLA-A2-restricted peptide epitopes tend to be poorly immunogenic in HLA-A2-transgenic mice. This finding was confirmed in HLA-A2-transgenic mice in-fected with a recombinant vaccinia virus expressing hepatitis C virus proteins. We examined the precursor frequency of epitope-specific naive CD8＋ T cells in HLA-A2-transgenic and conventional C57BL/6 mice and found that the poor im-munogenicity of HLA-A2-restricted peptide epitopes is re-lated to the paucity of naive CD8＋ T-cell precursors in HLA-A2-transgenic mice. These results provide direction for the improvement of mouse models to study epitope reper-toires and the immunodominance of human T-cell responses.
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KEYWORD
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CD8＋ T cells, T cell immunogenicity, T cell epit-ope, Major histocompatibility complex class I, HLA-A2, Naive T cell precursor
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